Lab

NEXT GENERATION SEQUENCING TECHNOLOGIES 

Ion Torrent Sequencing (Life Technologies Inc.)

PGMPGM chips

Ion Torrent is different from other NGS sequencers, because it does not make use of optical signals. Instead, it is a very complicated pH meter: it registers the change in electrical current that comes when the  addition of a dNTP to a DNA polymer releases an H+ ion .

ion1ion2

The input DNA or RNA is fragmented to ~200bp.   Adaptors are added and one molecule is placed onto a bead. The molecules are amplified on the bead by emulsion PCR. Each bead is placed into a single well on a chip.

Ion flow

The chip is flooded with a single species of dNTP, along with buffers and polymerase, one NTP at a time. The pH is detected is each of the wells, as each H+ ion released will decrease the pH. The changes in pH allow us to determine if that base, and how many thereof, was added to the sequence read.

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Laboratory of Genomic Diversity